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From: RonO <rokimoto557@gmail.com>
Newsgroups: talk.origins
Subject: Transposase evolved to insert DNA at specific locations
Date: Sat, 17 May 2025 10:53:44 -0500
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https://www.science.org/content/article/jumping-gene-enzyme-can-make-big-precise-changes-human-dna

An enzyme usually used by bacterial transposons to move from one place 
to another in the bacterial genome has been modified and evolved to 
insert large pieces of DNA into specific locations in mammalian genomes. 
  It uses the guide RNA of CRISPR to identify specific genomic sequence 
and they evolved the enzyme in order to improve the rate that it would 
insert sequence at the guide RNA location.  They used an in vivo 
selection environment where bacteria with the the enzyme had to survive 
viral infection.  They could survive viral infection if the enzyme could 
perform specific functions in the bacterial cell, so random mutations in 
the enzyme were selected for improved ability to perform the required 
duties.  They ended up with an enzyme that was much better than what 
they started with, and it could be useful for inserting large gene 
length segments of DNA into the human genome for fixing genetic defects.

Ron Okimoto